Publications

TUCCA has published 44 peer-reviewed papers about cultivated meat, cellular agriculture, and alternative proteins. If you are having trouble accessing the full text of a paper, please reach out. 

2025

Gordon, E. et al. Biomaterials in cellular agriculture and plant-based foods for the future. Nature Review Materials (2025). 
https://doi.org/10.1038/s41578-025-00800-7 

Letcher, S. et al. Establishment & Characterization of a Non-Adherent Insect Cell Line for Cultivated Meat. Scientific Reports (2025).
https://doi.org/10.1038/s41598-025-86921-z 

Dolgin, J. et al. Microbiral lysates as low-cost serum replacements in cellular agriculture media formulation. Food Research International (2025).
https://doi.org/10.1101/2024.10.28.620756 

2024

Li, X. et al. Fiber-based Biomaterial Scaffolds for Cell Support towards the Production of Cultivated Meat. Acta Biomaterialia (2024). 
https://doi.org/10.1016/j.actbio.2024.11.006

Riquelme-Guzmán, C. et al. Unlocking the potential of cultivated meat through cell line engineering. iScience (2024).
https://doi.org/10.1016/j.isci.2024.110877

Gerber, S. et al. Peanut hulls, an underutilized nutritious culinary ingredient: valorizing food waste for global food, health, and farm economies—a narrative review. Frontiers in Nutrition (2024). 
https://doi.org/10.3389/fnut.2024.1453315

Tuse, D. et al. A review and outlook on expression of animal proteins in plants. Frontiers in Plant Science (2024). 
https://doi.org/10.3389/fpls.2024.1426239

Guessous, G. et al. Disentangling the Web: An Interdisciplinary Review on the Potential and Feasibility of Spider Silk Bioproduction. ACS Biomaterials Science & Engineering (2024). 
 https://doi.org/10.1021/acsbiomaterials.4c00145 

Goswami, M. et al. Cell-cultivated aquatic food products: emerging production systems for seafood. Journal of Biological Engineering (2024).
https://doi.org/10.1186/s13036-024-00436-1 

Mazzaferro, L. et. al. Fouling resistance of novel amphiphilic polyampholyte membranes with varying hydrophobivity towards common foulants and cell culture media. Separation and Purification Technology (2024). 
https://doi.org/10.1016/j.seppur.2024.129090 

Nikkhah, A. et. al. Life cycle assessment of Beefy-9 and Beefy-R serum-free culture media for cell-cultivated beef production. Sustainable Production and Consumption (2024). 
https://doi.org/10.1016/j.spc.2024.07.023

Lew, E. et. al. Chemical and sensory analyses of cultivated pork fat tissue as a flavor enhancer for meat alternatives. Scientific Reports (2024). 
https://doi.org/10.1038/s41598-024-68247-4  

Joyce, C. et al. Methods to screen the adhesion of fish cells on plant-, algal- and fungal-derived biomaterials. ACS Applied Materials and Interfaces (2024). https://doi.org/10.1021/acsami.4c06543

Giglio, F. et al. A Glance into the Near Future: Cultivated Meat from Mammalian and Insect Cells. Small Science (2024). 
https://doi.org/10.1002/smsc.202400122 

Lim, T. et al. Development of serum-reduced medium for mackerel muscle cell line cultivation. ACS Sustainable Chemistry & Engineering (2024). https://doi.org/10.1021/acssuschemeng.4c03345 

Ovissipour, R. et al. Cell-based fish production case study for developing a food safety plan. Heliyon (2024). 
https://doi.org/10.1016/j.heliyon.2024.e33509

Gerber, S. et al. Marketing plant-based versus animal-sourced foods in online grocery stores: A comparative content analysis of sustainability and other product claims in the United States. Business Strategy and the Environment (2024). 
https://doi.org/10.1002/bse.3717 

Banavar, A. et al. Decellularized banana leaves: eco-friendly scaffolds for cell-based seafood. Frontiers in Sustainable Food Systems (2024). https://doi.org/10.3389/fsufs.2024.1341151

Stout, A. et al. Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat production. Cell Reports Sustainability (2024). https://doi.org/10.1016/j.crsus.2023.100009

Yao, Y. et al. Cultivated Meat from Aligned Muscle Layers and Adipose Layers Formed from Glutenin Films. ACS Biomaterials Science & Engineering (2024).
https://doi.org/10.1021/acsbiomaterials.3c01500

2023

Yuen, J. et al. Optimization of Culture Media and Cell Ratios for 3D In Vitro Skeletal Muscle Tissues with Endothelial Cells. ACS Biomaterials Science & Engineering (2023). 
https://doi.org/10.1021/acsbiomaterials.3c00358 

Sharma, A. et al. Multiplexed Sensing Probe for Bioreactors for Cellular Agriculture. IEEE Sensors Letters (2023).
10.1109/LSENS.2023.3300799

Trinidad, K. et al. Environmental life cycle assessment of recombinant growth factor production for cultivated meat applications. Journal of Cleaner Production (2023).
https://doi.org/10.1016/j.jclepro.2023.138153

Nikkhah, A. et al. Using artificial intelligence to optimize reduced-serum formulations for cultivated meat. Science of the Total Environment (2023).
https://doi.org/10.1016/j.scitotenv.2023.164988

Stout, A. et al. Immortalized Bovine Satellite Cells for Cultured Meat Applications. ACS Synthetic Biology (2023).
https://doi.org/10.1021/acssynbio.3c00216

John, Y. et al. Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications. eLife (2023). 
https://doi.org/10.7554/eLife.82120

Saad, M. et al. Continuous fish muscle cell line with capacity for myogenic and adipogenic-like phenotypes. Scientific Reports (2023).
https://doi.org/10.1038/s41598-023-31822-2.

Stout, A. et al. A Beefy-R culture medium: replacing albumin with rapeseed protein isolates. Biomaterials (2023).
https://doi.org/10.1016/j.biomaterials.2023.122092.

2022

Xiang, N. et al. Edible films for cultivated meat production. Biomaterials (2022). 
https://doi.org/10.1016/j.biomaterials.2022.121659.

Xiang, N. et al. 3D porous scaffolds from wheat glutenin for cultured meat applications. Biomaterials (2022). 
https://doi.org/10.1016/j.biomaterials.2022.121543

Stout, A. et al. Cultured meat: Creative solutions for a cell biological problem. Trends in Cell Biology (2022).
https://doi.org/10.1016/j.tcb.2022.10.002

Stout, A. et al. Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat. Communications Biology (2022).
https://doi.org/10.1038/s42003-022-03423-8

Letcher, S. et al. In vitro Insect Fat Cultivation for Cellular Agriculture Applications. ACS Biomaterials Science & Engineering (2022).
https://doi.org/10.1021/acsbiomaterials.2c00093

2021

Yuen, J. et al. Perspectives on Scaling Production of Adipose Tissue for Food Applications. Biomaterials (2021).
https://dx.doi.org/10.1016/j.biomaterials.2021.121273.

Stout, A. et al. Simple and Effective Serum-Free Medium for Sustained Expansion of Bovine Satellite Cells for Cell Cultured Meat. bioRxiv (2021). https://dx.doi.org/10.1101/2021.05.28.446057.

2020

Fish, K. et al. Prospects and Challenges for Cell-Cultured Fat as a Novel Food Ingredient. Trends in Food Science & Technology (2020). https://dx.doi.org/10.1016/j.tifs.2020.02.005.

Post, M. et al. Scientific, Sustainability and Regulatory Challenges of Cultured Meat. Nature Food  (2020)
https://dx.doi.org/10.1038/s43016-020-0112-z.

Stout, A. et al. Engineering Carotenoid Production in Mammalian Cells for Nutritionally Enhanced Cell-Cultured Foods. Metabolic Engineering (2020). https://dx.doi.org/10.1016/j.ymben.2020.07.011.

Rubio, N. et al. Plant-Based and Cell-Based Approaches to Meat Production. Nature communications (2020).
https://dx.doi.org/10.1038/s41467-020-20061-y.

Rubio, N. et al. Biofabrication with Insect Cells.Trends in Entomology (2020)
Article_pdf.asp.researchtrends.net.

2019

Rubio, N. et al. Cell-Based Fish: A Novel Approach to Seafood Production and an Opportunity for Cellular Agriculture. Frontiers in Sustainable Food Systems (2019).
https://dx.doi.org/10.3389/fsufs.2019.00043.

Rubio, N. et al. Possibilities for Engineered Insect Tissue as a Food Source. Frontiers in Sustainable Food Systems (2019). https://dx.doi.org/10.3389/fsufs.2019.00024.

Rubio, N. et al. In Vitro Insect Muscle for Tissue Engineering Applications. ACS Biomaterials Science & Engineering (2019). https://dx.doi.org/10.1021/acsbiomaterials.8b01261.

Simsa, R. et al. Extracellular Heme Proteins Influence Bovine Myosatellite Cell Proliferation and the Color of Cell-Based Meat. Foods (2019). https://dx.doi.org/10.3390/foods8100521.

2014

Baryshyan, A. Self-Assembled Insect Muscle Bioactuators with Long Term Function under a Range of Environmental Conditions. RSC Advances (2014). https://dx.doi.org/10.1039/C4RA08438A.

2012

Baryshyan, A. Isolation and Maintenance-Free Culture of Contractile Myotubes from Manduca Sexta Embryos. PloS one (2012). https://dx.doi.org/10.1371/journal.pone.0031598.