Publications

2023

Trinidad, K. R., Ashizawa, R., Nikkhah, A., Semper, C., Casolaro, C., Kaplan, D. L., ... & Blackstone, N. T. (2023). Environmental life cycle assessment of recombinant growth factor production for cultivated meat applications. Journal of Cleaner Production, 419, 138153.
https://doi.org/10.1016/j.jclepro.2023.138153

Nikkhah A, Rohani A, Zarei M, Kulkarni A, Batarseh FA, Blackstone NT, Ovissipour R. Toward sustainable culture media: Using artificial intelligence to optimize reduced-serum formulations for cultivated meat. Sci Total Environ. 2023 Jun 19;894:164988. Epub ahead of print. PMID: 37343855.
https://doi.org/10.1016/j.scitotenv.2023.164988

Stout AJ, Arnett MJ, Chai K, Guo T, Liao L, Mirliani AB, Rittenberg ML, Shub M, White EC, Yuen JSK Jr, Zhang X, Kaplan DL. Immortalized Bovine Satellite Cells for Cultured Meat Applications. ACS Synth Biol. 2023 May 5. Epub ahead of print. PMID: 37146268.
https://doi.org/10.1021/acssynbio.3c00216

John Se Kit Yuen Jr, Michael K Saad, Ning Xiang, Brigid M Barrick, Hailey DiCindio, Chunmei Li, Sabrina W Zhang, Miriam Rittenberg, Emily T Lew, Kevin Lin Zhang, Glenn Leung, Jaymie A Pietropinto, David L Kaplan (2023) Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications eLife 12:e82120
https://doi.org/10.7554/eLife.82120

Saad, Michael K., John SK Yuen Jr, Connor M. Joyce, Xinxin Li, Taehwan Lim, Talia L. Wolfson, Justin Wu et al. "Continuous fish muscle cell line with capacity for myogenic and adipogenic-like phenotypes." Scientific Reports 13, no. 1 (2023): 5098. 
https://doi.org/10.1038/s41598-023-31822-2.

Stout, Andrew J., Miriam L. Rittenberg, Michelle Shub, Michael K. Saad, Addison B. Mirliani, James Dolgin, and David L. Kaplan. "A Beefy-R culture medium: replacing albumin with rapeseed protein isolates." Biomaterials (2023): 122092.
https://doi.org/10.1016/j.biomaterials.2023.122092.

2022

Xiang, N., Y. Yao, J. S. K. Yuen, A. J. Stout, C. Fennelly, R. Sylvia, A. Schnitzler, S. Wong and D. L. Kaplan. "Edible films for cultivated meat production." Biomaterials 287 (2022): 121659.
https://doi.org/10.1016/j.biomaterials.2022.121659.

Xiang, N., Yuen Jr, J. S., Stout, A. J., Rubio, N. R., Chen, Y., & Kaplan, D. L. (2022). 3D porous scaffolds from wheat glutenin for cultured meat applications. Biomaterials, 285, 121543. 
https://doi.org/10.1016/j.biomaterials.2022.121543

Stout, A. J., Kaplan, D. L., & Flack, J. E. (2022). Cultured meat: Creative solutions for a cell biological problem. Trends in Cell Biology.
https://doi.org/10.1016/j.tcb.2022.10.002

Stout, A. J., Mirliani, A. B., Rittenberg, M. L., Shub, M., White, E. C., Yuen, J. S. K., & Kaplan, D. L. (2022). Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat. Communications Biology, 5(1), 466.
https://doi.org/10.1038/s42003-022-03423-8

Letcher, S. M., Rubio, N. R., Ashizawa, R. N., Saad, M. K., Rittenberg, M. L., McCreary, A., Ali, A., Calkins, O. P., Trimmer, B. A., & Kaplan, D. L. (2022). In vitro Insect Fat Cultivation for Cellular Agriculture Applications. ACS Biomaterials Science & Engineering, 8(9), 3785–3796. https://doi.org/10.1021/acsbiomaterials.2c00093

2021

Yuen Jr, John S. K., Andrew J. Stout, N. Stephanie Kawecki, Sophia M. Letcher, Sophia K. Theodossiou, Julian M. Cohen, Brigid M. Barrick, Michael K. Saad, Natalie R. Rubio, Jaymie A. Pietropinto, Hailey DiCindio, Sabrina W. Zhang, Amy C. Rowat, and David L. Kaplan. "Perspectives on Scaling Production of Adipose Tissue for Food Applications." Biomaterials 280 (2021): 121273. https://dx.doi.org/10.1016/j.biomaterials.2021.121273.

Stout, Andrew J., Addison B. Mirliani, Eugene C. White, John S. K. Yuen, and David L. Kaplan. "Simple and Effective Serum-Free Medium for Sustained Expansion of Bovine Satellite Cells for Cell Cultured Meat." bioRxiv 5, no. 28 (2021): 446057. https://dx.doi.org/10.1101/2021.05.28.446057.

2020

Fish, Kyle D., Natalie R. Rubio, Andrew J. Stout, John S. K. Yuen, and David L. Kaplan. "Prospects and Challenges for Cell-Cultured Fat as a Novel Food Ingredient." Trends in Food Science & Technology 98 (2020): 53-67. https://dx.doi.org/10.1016/j.tifs.2020.02.005.

Post, Mark J., Shulamit Levenberg, David L. Kaplan, Nicholas Genovese, Jianan Fu, Christopher J. Bryant, Nicole Negowetti, Karin Verzijden, and Panagiota Moutsatsou. "Scientific, Sustainability and Regulatory Challenges of Cultured Meat." Nature Food 1, no. 7 (2020): 403-15. https://dx.doi.org/10.1038/s43016-020-0112-z.

Stout, Andrew J., Addison B. Mirliani, Erin L. Soule-Albridge, Julian M. Cohen, and David L. Kaplan. "Engineering Carotenoid Production in Mammalian Cells for Nutritionally Enhanced Cell-Cultured Foods." Metabolic Engineering 62 (2020): 126-37. https://dx.doi.org/10.1016/j.ymben.2020.07.011.

Rubio, Natalie R., Ning Xiang, and David L. Kaplan. "Plant-Based and Cell-Based Approaches to Meat Production." Nature communications 11, no. 1 (2020): 6276-76. https://dx.doi.org/10.1038/s41467-020-20061-y.

Rubio, Natalie, Naya McCartney, Barry Trimmer, and David Kaplan. "Biofabrication with Insect Cells." Trends in Entomology 16 (2020): 1-17. Article_pdf.asp.researchtrends.net.

2019

Rubio, Natalie, Isha Datar, David Stachura, David Kaplan, and Kate Krueger. "Cell-Based Fish: A Novel Approach to Seafood Production and an Opportunity for Cellular Agriculture." Frontiers in Sustainable Food Systems 3 (2019): 43. https://dx.doi.org/10.3389/fsufs.2019.00043.

Rubio, Natalie R., Kyle D. Fish, Barry A. Trimmer, and David L. Kaplan. "Possibilities for Engineered Insect Tissue as a Food Source." Review, Frontiers in Sustainable Food Systems 3 (2019): 24. https://dx.doi.org/10.3389/fsufs.2019.00024.

Rubio, Natalie R., Kyle D. Fish, Barry A. Trimmer, and David L. Kaplan. "In Vitro Insect Muscle for Tissue Engineering Applications." ACS Biomaterials Science & Engineering 5, no. 2 (2019): 1071-82. https://dx.doi.org/10.1021/acsbiomaterials.8b01261.

Simsa, Robin, John Yuen, Andrew Stout, Natalie Rubio, Per Fogelstrand, and David L. Kaplan. "Extracellular Heme Proteins Influence Bovine Myosatellite Cell Proliferation and the Color of Cell-Based Meat." Foods (Basel, Switzerland) 8, no. 10 (2019): 521. https://dx.doi.org/10.3390/foods8100521.

2014

Baryshyan, A. L., L. J. Domigan, B. Hunt, B. A. Trimmer, and D. L. Kaplan. "Self-Assembled Insect Muscle Bioactuators with Long Term Function under a Range of Environmental Conditions." RSC Advances 4, no. 75 (2014): 39962-68. https://dx.doi.org/10.1039/C4RA08438A.

2012

Baryshyan, Amanda L., William Woods, Barry A. Trimmer, and David L. Kaplan. "Isolation and Maintenance-Free Culture of Contractile Myotubes from Manduca Sexta Embryos." PloS one 7, no. 2 (2012): e31598-e98. https://dx.doi.org/10.1371/journal.pone.0031598.